The aim of the MIRAGE project is to improve the quality of glycomics data in the scientific literature by focusing on the development of publication and data reporting guidelines. The MIRAGE-LC module covers all aspects of instrument setup and modality of data handling and manipulation cross-linked with other MIRAGE recommendations.
This section provides an example Human IgG glycan dataset reported in compliance with the MIRAGE Guidelines For each glycan the elution time and panel of sequential exoglycosidases (ABS, α2–3,6,8,9 sialidase; BTG, β1-3,4 galactosidase; BKF, α1-2,3,4,6 fucosidase) used to quantitate and confirm structural assignments is provided using a PGC-LC-MS/MS workflow.
No. | Structure | GlyTouCan | GlycoStore | RT (min) | Relative % Area | Exoglycosidase | m/z calc | m/z obs | MS2 |
---|---|---|---|---|---|---|---|---|---|
1 | G59471TH | GS734 | 29.8 | 0.38 | ABS+BTG+BKF | 759.8 | 759.7 | Y | |
2 | G96014TN | GS778 | 32.4 | 0.45 | ABS+BTG | 840.8 | 840.8 | N | |
3 | G62880PC | GS736 | 36.1 | 5.02 | ABS+BTG+BKF | 832.8 | 832.8 | Y | |
4 | G52231BF | GS826 | 37.8 | 0.27 | ABS | 1067.4 | 1067.4 | N | |
5 | G39213VZ | GS759 | 37.8 | 0.25 | ABS+BTG+BKF | 658.2 | 658.2 | N | |
6 | G45686NF | GS637 | 38.1 | 4.67 | ABS+BTG | 913.8 | 913.8 | Y | |
7 | G53000RQ | GS943 | 39.5 | 1.52 | ABS+BTG | 994.9 | 994.9 | Y | |
8 | G89993FE | GS632 | 39.9 | 0.96 | ABS+BTG | 739.3 | 739.3 | N | |
9 | G50779LX | GS648 | 39.9 | 0.14 | ABS | 1212.9 | 1212.9 | N | |
10 | G21393DK | GS100026 | 40.1 | 0.79 | ABS | 1059.4 | 1059.4 | Y | |
11 | G66937TJ | GS937 | 41 | 0.65 | ABS+BTG | 739.3 | 739.3 | N | |
12 | G15826PZ | GS825 | 42.1 | 1.20 | ABS | 1140.4 | 1140.4 | Y | |
13 | G36191CD | GS235 | 42.9 | 1.03 | ABS+BTG | 820.3 | 820.3 | Y | |
14 | G09629PQ | GS100025 | 43.4 | 0.45 | ABS | 1285.9 | 1285.9 | Y | |
15 | G69812OF | GS735 | 43.8 | 22.28 | ABS+BTG+BKF | 731.3 | 731.2 | Y | |
16 | G18609BT | GS941 | 44.8 | 0.53 | ABS | 965.8 | 965.8 | Y | |
17 | G09782JM | GS630 | 45.6 | 19.88 | ABS+BTG | 812.3 | 812.3 | Y | |
18 | G84467IZ | GS100022 | 46 | 0.27 | ABS | 1111.4 | 1111.4 | N | |
19 | G09782JM | GS630 | 46.3 | 12.64 | ABS+BTG | 812.3 | 812.3 | Y | |
20 | G33284MM | GS816 | 47.7 | 2.06 | ABS | 957.8 | 957.8 | Y | |
21 | G28826SH | GS731 | 47.9 | 15.81 | ABS+BTG | 893.3 | 893.3 | Y | |
22 | G62689JX | GS683 | 49.1 | 7.79 | ABS | 1038.9 | 1038.9 | Y | |
23 | G83594ZB | GS100017 | 50 | 0.98 | ABS | 1184.4 | 1184.4 | Y |
Peak lists available for download here
Gradient: (85 min) 100% A for 8 min, 5-35% B (8-53 min), 35-100% B (53-73 min), 100% B (73-78 min), 100% A (79-83 min) 2 min post run 100% A