No. Structure GlyTouCan GlycoStore RT (min) Relative % Area Exoglycosidase m/z calc m/z obs MS2
21 G28826SH GS731 47.9 15.81 ABS+BTG 893.3 893.3 Y

MS2 Spectra

Supporting MS2

Peak lists available for download here

Glycoprotein Standard

Human IgG (Sigma)

Equipment

Column: Thermo Hypercarb PGC Column Dimensions: 3 μm, 100 mm × 180 μm (i.d.) Mass Spec: Agilent 6330 XCT Ultra Ion Trap LC: Agilent 1260 series HPLC

Properties of the Chromatographic Run

Gradient: 85 min Flow Rate: 2 uL/min Injection: 4 µl (8 µl injection loop)

Mobile Phase

A: 10 mM Ammonium bicarbonate B: 45% Acetonitrile in 10 mM Ammonium bicarbonate

Extended Notes

Gradient: (85 min) 100% A for 8 min, 5-35% B (8-53 min), 35-100% B (53-73 min), 100% B (73-78 min), 100% A (79-83 min) 2 min post run 100% A