Glycan Name Time (mins) Rel. to M5

M4A1B 28.85 15.24

A2B 29.81 14.28

M1 30.38 13.71

M4A2B 30.96 13.13

M5A1B 31.0 13.09

A3B 31.43 12.66

A2BG(4)1 32.51 11.58

M5A2B 32.7 11.39

M4A1BG(4)1 34.43 9.66

M4BA1G(4)1 34.43 9.66

A2BG(4)2 34.63 9.46

A2BG(4)1S(6)1 35.43 8.66

M4A2BG(4)1 35.5 8.59

A3BG(4)1 35.92 8.16

M5A2BG(4)1 36.04 8.05

F(6)A2B 36.12 7.97

M7 D1 36.68 7.41

M8 36.96 7.13

M9 37.43 6.66

A2 37.5 6.59

A2BG(4)2S(6)1 37.79 6.3

M6 38.04 6.04

M4 D1 38.07 6.02

F(6)A2BG(4)1 38.21 5.88

A2G(4)2F(3)2 38.48 5.61

M7 D2 38.77 5.32

M4 D2 39.41 4.67

F(6)A2BG(4)2 39.53 4.56

A2[6]G(4)1 39.89 4.2

F(6)A2BG(4)1S(6)1 39.99 4.1

M4A1G(4)1S(6)1 40.12 3.96

A2G(4)2F(3)1 40.73 3.36

A2[3]G(4)1 40.97 3.12

M5A1G(4)1 41.03 3.06

M3 41.22 2.86

A1G(4)1S(6)1 41.44 2.65

A3 41.88 2.21

F(6)A2BG(4)2S(6)1 41.9 2.19

A1G(4)1 42.7 1.39

A2G(4)2 42.76 1.33

A2G(4)1S(6)1 43.01 1.07

M5A1G(4)1S(6)1 43.13 0.96

F(6)A2BG(4)2S(6)2 43.49 0.6

F(6)A2 43.76 0.33

F(6)A2G(4)2F(3)2 44.02 0.07

M5 44.09 0.0

F(6)A1G(4)1 44.4 -0.31

A2G(4)2S(6)1 44.62 -0.53

A1[6] 44.65 -0.56

F(6)M4A1G(4)1S(6)1 44.87 -0.78

F(6)A2[6]G(4)1 45.57 -1.48

A2G(4)2S(6)2 45.59 -1.5

F(6)A2G(4)2F(3)1 45.75 -1.66

A3G(4)3S1 46.1 -2.01

A3G3S1 46.1 -2.01

F(6)M5A1G(4)1 46.2 -2.11

F(6)A2[3]G(4)1 46.22 -2.13

A3G(4)3F(3)1 46.4 -2.31

F(6)A1G(4)1S(6)1 46.47 -2.38

F(6)A1G1S(6)1 46.47 -2.38

F(6)A2G(4)2F1 47.27 -3.18

A3G(4)3F1S(6)1 47.4 -3.31

A3G(4)3S(6)1 47.55 -3.46

F(6)A2G(4)2F(3)1S(6)1 47.68 -3.59

F(6)A2G(4)1S(6)1 47.7 -3.61

F(6)A2G(4)2 47.76 -3.67

A2G(4)2F(3)1S1 48.55 -4.46

A2G(4)2F(3)1S2 48.57 -4.48

A3G(4)3F1S2 49.03 -4.94

F(6)A2G(4)2S(6)1 49.07 -4.98

F(6)A2G(4)2S(6)2 50.17 -6.08

A3G(4)3S(6)2 50.23 -6.14

F(6)A2G(4)2F2S1 50.39 -6.3

M5A1G(4)1S1 50.55 -6.46

A4G(4)4 50.8 -6.71

F(6)A2G(4)2S1 51.0 -6.91

A3G(4)3S(6)3 52.03 -7.94

A2G(4)2F(2)2S1 52.52 -8.43

A2G(4)2S(3,6)2 52.7 -8.61

A3G(4)3S2 53.67 -9.58

A3G(4)3S(3,3,6)3 53.8 -9.71

A3G3S(3,3,6)3 53.8 -9.71

A4G(4)4S4 53.97 -9.88

A4G(4)4S3 54.37 -10.28

A4G(4)4F1S4 55.6 -11.51

A4G(4)4F1S3 58.03 -13.94

A3G(4)3S(3)3 60.53 -16.44

Retention Time Viewer

Graphical View

Glycoprotein Standards

Human IgG Human IgA RNAseB Ovalbumn Alpha-1-Acid Glycoprotein Lactoferrin

Information

Column: Hypercarb PGC column Gradient: 85 min gradient of 0–45% (v/v) acetonitrile in 10 mM ammonium bicarbonate Flow Rate: 2 uL/min Mass Spec: Agilent XCT Ultra LC: Agilent 1260 series HPLC

Notes

The following MS settings were used: drying gas temperature: 300°C, drying gas flow: 6 L/min, nebulizer gas: 12 psi, skimmer, trap drive and capillary exit were set at ‐40 V, ‐99.1 V and ‐166 V, respectively