Glycan Name Time (mins)

A2BG(4)2 34.3

A2BG(4)2 34.8


Graphical View

Glycoprotein Standards

Human IgA Human IgG RNAseB Ovalbumn Alpha-1-Acid Glycoprotein Lactoferrin


Column: Hypercarb PGC column Gradient: 85 min gradient of 0–45% (v/v) acetonitrile in 10 mM ammonium bicarbonate Flow Rate: 2 uL/min Mass Spec: Agilent XCT Ultra LC: Agilent 1260 series HPLC


The following MS settings were used: drying gas temperature: 300°C, drying gas flow: 6 L/min, nebulizer gas: 12 psi, skimmer, trap drive and capillary exit were set at ‐40 V, ‐99.1 V and ‐166 V, respectively