Glycan Name Time (mins)

A2[6]G(4)1 39.7

A2[6]G(4)1 39.7

A2[6]G(4)1 39.9

A2[6]G(4)1 40.0

Spectra

Graphical View

Glycoprotein Standards

Lactoferrin Human IgG Human IgA RNAseB Ovalbumn Alpha-1-Acid Glycoprotein

Information

Column: Hypercarb PGC column Gradient: 85 min gradient of 0–45% (v/v) acetonitrile in 10 mM ammonium bicarbonate Flow Rate: 2 uL/min Mass Spec: Agilent XCT Ultra LC: Agilent 1260 series HPLC

Notes

The following MS settings were used: drying gas temperature: 300°C, drying gas flow: 6 L/min, nebulizer gas: 12 psi, skimmer, trap drive and capillary exit were set at ‐40 V, ‐99.1 V and ‐166 V, respectively