Glycan Name Time (mins)

A2G(4)2F(3)1 40.1

A2G(4)2F(3)1 40.3

A2G(4)2F(3)1 40.5

A2G(4)2F(3)1 40.8

A2G(4)2F(3)1 40.9

A2G(4)2F(3)1 41.2

A2G(4)2F(3)1 41.3

Spectra

Graphical View

Glycoprotein Standards

Lactoferrin Human IgG Human IgA RNAseB Ovalbumn Alpha-1-Acid Glycoprotein

Information

Column: Hypercarb PGC column Gradient: 85 min gradient of 0–45% (v/v) acetonitrile in 10 mM ammonium bicarbonate Flow Rate: 2 uL/min Mass Spec: Agilent XCT Ultra LC: Agilent 1260 series HPLC

Notes

The following MS settings were used: drying gas temperature: 300°C, drying gas flow: 6 L/min, nebulizer gas: 12 psi, skimmer, trap drive and capillary exit were set at ‐40 V, ‐99.1 V and ‐166 V, respectively